| Registro completo |
| Provedor de dados: |
OAK
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| País: |
Japan
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| Título: |
Effect of Zona Incision by Piezo-Micromanipulator (ZIP) on In Vitro Fertilization in 21 Transgenic Mice Lines
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| Autores: |
KAWASE, Yosuke
TACHIBE, Takanori
HANI, Toshio
TATEISHI, Hiromi
JISHAGE, Kou-ichi
SUZUKI, Hiroshi
鈴木, 宏志
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| Data: |
2009
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| Ano: |
2009
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| Palavras-chave: |
Frozen-thawed spermatozoa
IVF
ZIP
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| Resumo: |
Zona incision using a piezo-micromanipulator (ZIP) has been demonstrated to be effective for in vitro fertilization (IVF) using cryopreserved C57BL/6 spermatozoa. In this study, ZIP oocytes inseminated with frozen-thawed genetically modified C57BL/6J or FVB mice spermatozoa (21 lines) showed fertilization rates of 22-75% and live fetus rates of 8-49%. In 6 of the lines, the fertilization rates for oocytes compared with ZIP (42-75%) were significantly higher than that of nontreated oocytes (0-50%). Using only 90 oocytes for IVF with ZIP, 5 breeding pairs were produced from cryopreserved genetically modified mice spermatozoa. Our results indicate that application of the ZIP technique is effective for IVF using cryopreserved genetically modified mouse spermatozoa.
http://www.jstage.jst.go.jp/article/expanim/58/4/58_415/_article/-char/ja/
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| Idioma: |
Inglês
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| Identificador: |
http://ir.obihiro.ac.jp/dspace/handle/10322/3017
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| Editor: |
日本実験動物学会
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| Formato: |
application/pdf
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| Direitos: |
日本実験動物学会
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